Construction of porcine reproductive and respiratory syndrome virus m protein gene vaccine and immunological experiment on mice 蛋白基因疫苗的構建及在小鼠的免疫試驗
The sequence 56 analysis shows that there is a long open reading frame in it which encode a protein of 364 animo acid residues . the m protein include 47 basic amino acid , 30 acidic amino acid , which cause a basic estimated pi of 9 . 7 . phylogenetic tree based on ndv matrix protein gene shows that the b95 and v4 has the most close relationship than other ndv strain 關于ndvm蛋白國內未見有報道,國外對此蛋白的研究也十分有限,本文克隆出了ndv株b95m蛋白基因的全序列,為m蛋白的表達及進一步研究其在病毒復制與致病中的作用機理打下了基礎。